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Related Experiment Video

Updated: Jan 30, 2026

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
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Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

Published on: October 15, 2014

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A rapid method for post-antibiotic bacterial susceptibility testing.

Andrew A Heller1,2, Dana M Spence2,3

  • 1Department of Chemistry, Michigan State University, East Lansing, Michigan, United States of America.

Plos One
|January 11, 2019
PubMed
Summary
This summary is machine-generated.

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This study introduces a rapid antibiotic susceptibility test measuring ATP release and optical density. The ATP:OD600 ratio increase indicates antibiotic efficacy, offering a faster alternative for bacterial infection treatment and drug discovery.

Area of Science:

  • Microbiology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Antibiotic susceptibility testing is crucial for effective treatment and monitoring drug resistance.
  • Current methods can be time-consuming, driving demand for faster diagnostic tools.
  • Bacterial ATP release and optical density are indicators of metabolic activity and cell lysis.

Purpose of the Study:

  • To develop and validate a rapid antibiotic susceptibility test.
  • To utilize chemiluminescent ATP determination and optical density (OD600) measurements.
  • To establish the ATP:OD600 ratio as a quantitative indicator of antibiotic efficacy.

Main Methods:

  • Bacteria were cultured to a steady growth phase.
  • Antibiotics were added, and ATP release and OD600 were measured.

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Last Updated: Jan 30, 2026

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
11:25

Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing

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Stress-induced Antibiotic Susceptibility Testing on a Chip
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  • The ratio of ATP to OD600 was calculated to assess antibiotic effect.

    • Main Results:

    • A significant increase in the ATP:OD600 ratio was observed when susceptible bacteria (E. coli, B. subtilis) were treated with effective antibiotics.
    • This increase was measurable within 20 minutes of antibiotic addition.
    • The method demonstrated utility for both Gram-negative (E. coli) and Gram-positive (B. subtilis) bacteria.

    Conclusions:

    • The ATP:OD600 ratio provides a rapid and quantitative measure of antibiotic susceptibility.
    • This novel method has potential applications in clinical diagnostics and antibiotic drug discovery.
    • The technique offers a faster and more precise alternative to existing susceptibility testing methods.