The effect of the placental DROSHA rs10719 and rs6877842 polymorphisms on PE susceptibility and mRNA expression

Related Experiment Video

Updated: Jan 30, 2026

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

12.2K

Mahnaz Rezaei1, Abbas Mohammadpour-Gharehbagh1,2, Mehrnaz Narooei-Nejad3

  • 1Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.

Journal of Human Hypertension
|January 12, 2019
PubMed

Insights

Preeclampsia (PE) risk may be linked to the DROSHA rs10719 gene variant, particularly in a recessive model. DROSHA mRNA expression was lower in placentas from PE patients, especially those with the rs10719CC genotype.

Area of Science:

  • Genetics
  • Obstetrics
  • Molecular Biology

Background:

  • MicroRNA biosynthesis is implicated in Preeclampsia (PE) pathogenesis.
  • MicroRNA processing enzymes, like DROSHA, may influence PE predisposition.

Purpose of the Study:

  • To investigate the association between DROSHA gene polymorphisms (rs10719 and rs6877842) and mRNA expression in the placenta of PE patients and controls.
  • To evaluate the role of DROSHA variants in PE susceptibility.

Main Methods:

  • Genotyping of DROSHA rs10719 and rs6877842 polymorphisms in 110 PE women and 115 controls.
  • Analysis of DROSHA mRNA expression in placental tissues.
  • Statistical analysis to assess associations between genotypes, haplotypes, mRNA levels, and PE risk.

Main Results:

  • No overall association between DROSHA rs10719/rs6877842 alleles/genotypes and PE susceptibility.
  • DROSHA rs10719 was associated with increased PE risk in a recessive model.
  • Combined CC/GG genotypes of rs10719 and rs6877842 were linked to higher PE risk.
  • DROSHA mRNA expression was downregulated in PE placentas.
  • Decreased DROSHA mRNA expression was observed in placentas with the rs10719CC genotype.

Conclusions:

  • The placental DROSHA rs10719 polymorphism, specifically in the recessive model, may be a risk factor for PE susceptibility.
  • Combined genotypes (CC/GG) of DROSHA rs10719 and rs6877842 could increase PE risk.
  • Downregulation of DROSHA expression in preeclamptic placentas, particularly in carriers of the rs10719CC genotype, warrants further investigation.

More Related Videos

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks09:33

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Published on: March 29, 2018

10.3K
In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells10:07

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

Published on: November 13, 2014

25.7K

Related Concept Videos

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

3.5K
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.0K
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.5K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
Gonadal and Placental Hormones01:24

Gonadal and Placental Hormones

The gonads, namely the testes in males and the ovaries in females, are pivotal in producing gonadal hormones that orchestrate the intricate processes of sexual development and reproduction.
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair...
3.1K