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Related Experiment Video

Updated: Jan 30, 2026

Measurement of Larval Activity in the Drosophila Activity Monitor

Measurement of Larval Activity in the Drosophila Activity Monitor

Published on: April 30, 2015

Antimalarial Activity of Piperine.

Artitaya Thiengsusuk¹, Phunuch Muhamad¹, Wanna Chaijaroenkul²

¹Drug Discovery and Development Center, Thammasat University (Rangsit Campus), Pathumtani 12121, Thailand.

Journal of Tropical Medicine

|January 12, 2019

Summary

Piperine, derived from Piper chaba, shows significant antimalarial activity against Plasmodium falciparum, including drug-resistant strains. This natural compound offers a promising, low-resistance risk alternative for malaria treatment.

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Area of Science:

Pharmacology
Tropical Medicine
Molecular Biology

Background:

Malaria, caused by Plasmodium falciparum, is a major global health issue, particularly in tropical regions.
Artemisinin resistance in Southeast Asia necessitates the urgent development of new antimalarial drugs.
Previous research indicated antimalarial activity of Piper chaba fruit against P. falciparum.

Purpose of the Study:

To evaluate the antimalarial efficacy of piperine, a key compound from Piper chaba fruit.
To assess piperine's activity against both chloroquine-sensitive (3D7) and chloroquine-resistant (K1) P. falciparum clones.
To investigate piperine's impact on P. falciparum drug resistance gene expression.

Main Methods:

Antimalarial activity was quantified using a SYBR green-I-based assay to determine median IC50 values.
Parasite morphology was examined via light microscopy with Giemsa staining after piperine exposure.
Real-time PCR was employed to analyze the expression of P. falciparum drug resistance genes (pfcrt, pfmdr1, pfmrp1).

Main Results:

Piperine demonstrated median IC50 values of 111.5 μM against 3D7 and 59 μM against K1 P. falciparum clones.
Significant alterations in parasite morphology were observed within 48 hours of piperine treatment.
Piperine did not affect the expression of pfcrt, pfmdr1, and pfmrp1 genes, which are linked to antimalarial drug resistance.

Conclusions:

Piperine exhibits potent antimalarial activity against P. falciparum, including resistant strains.
The compound shows a low risk of inducing drug resistance due to its lack of effect on key resistance genes.
Piperine represents a promising candidate for further development as a novel antimalarial therapeutic agent.