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Updated: Jan 30, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
Fluorescent Light-up Probe for the Detection of Protein Aggregates
Raquel Nunes da Silva1,2, Catarina C Costa1, Maria J G Santos1
1Department of chemistry and QOPNA, University of Aveiro, Campus of Santiago, Aveiro, Portugal.
Abstract:
A fluorescent dye was decorated with water-soluble pyridinium groups in order to be applied in the detection of cyclodextrins or DNA. The dye displays an enhancement of its emission intensity when the internal rotations are restricted due to the formation of an inclusion complex with cyclodextrins or upon interaction with DNA. In vivo, the fluorescent probe can stain protein aggregates with a selectivity comparable to the widely used Proteostat®.
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