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Solid Lipid Nanoparticles SLNs for Intracellular Targeting Applications
Published on: November 17, 2015
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Amphotericin B loaded solid lipid nanoparticles (SLNs) and nanostructured lipid carrier (NLCs): physicochemical and
Phatsawee Jansook1, Zoltán Fülöp2, Garnpimol C Ritthidej1
1a Faculty of Pharmaceutical Sciences , Chulalongkorn University , Pathumwan , Bangkok , Thailand.
Drug Development and Industrial Pharmacy
|January 12, 2019
Summary
This study developed novel lipid nanoparticles (SLNs and NLCs) for Amphotericin B (AmB), a potent antifungal. These lipid carriers, stabilized by poloxamer 407 and phospholipids, offer a less toxic alternative to conventional formulations.
Area of Science:
- Pharmaceutical Nanotechnology
- Drug Delivery Systems
- Antifungal Agents
Background:
- Amphotericin B (AmB) is a highly effective antifungal drug, but its clinical use is limited by severe toxicity associated with its conventional formulation.
- Lipid nanoparticle formulations have shown potential to reduce AmB toxicity while maintaining efficacy.
Purpose of the Study:
- To develop and characterize Amphotericin B (AmB) loaded solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs).
- To evaluate the stabilization of AmB lipid dispersions using various surfactants and phospholipids.
Main Methods:
- Formulation of SLNs and NLCs using different surfactants (Tween 20, Cremophor RH40, Poloxamer 407, Myrj 52) and phospholipids.
- Characterization using Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), Hot-Stage Microscopy (HSM), Powder X-ray Diffractometry (PXRD), and Proton Nuclear Magnetic Resonance (¹H-NMR).
Main Results:
- Poloxamer 407 (P407) was identified as the only effective surfactant for stabilizing AmB lipid dispersions.
- FT-IR confirmed no chemical reactions between AmB and formulation components.
- DSC, HSM, and PXRD data indicated molecular dispersion or amorphous state of AmB within the lipid matrix.
- ¹H-NMR revealed the formation of oil clusters in the presence of phospholipids.
Conclusions:
- Successfully developed AmB-loaded SLNs and NLCs stabilized by P407 and/or phospholipids.
- These lipid nanoparticles represent promising colloidal carriers for a safer Amphotericin B drug delivery system.
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