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Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells
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Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Cochlear Function in an Experimental Rat Model
Rahul Mittal1, Emre Ocak1, Angela Zhu1
1Department of Otolaryngology, Hearing Research Laboratory, University of Miami Miller School of Medicine, Miami, Florida.
Anatomical Record (Hoboken, N.J. : 2007)
|January 12, 2019
Summary
Transtympanic administration of bone marrow-derived mesenchymal stem cells (BM-MSCs) is safe for the inner ear. This non-surgical approach shows potential for otoprotection without immunosuppression in rats.
Area of Science:
- Regenerative Medicine
- Otolaryngology
- Stem Cell Biology
Background:
- Mesenchymal stem cells (MSCs) show therapeutic promise for various diseases.
- The efficacy of MSCs for cochlear insults and their safety in immunocompetent cochleas remain unclear.
- Previous studies often required immunosuppression or complex surgical delivery methods.
Purpose of the Study:
- To evaluate the safety and functional impact of non-surgically administered rodent bone marrow-derived MSCs (BM-MSCs) via transtympanic delivery.
- To assess the biocompatibility of BM-MSCs in the inner ear of immunocompetent rats.
- To determine potential adverse effects on the auditory system.
Main Methods:
- Rodent BM-MSCs were administered transtympanically into rats without immunosuppression.
- Auditory function was assessed using auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAE).
- Histopathological examination evaluated cochlear inflammation and edema.
Main Results:
- Transtympanic BM-MSC administration did not significantly alter hearing thresholds (ABR and DPOAE).
- No inflammatory leukocyte recruitment or edema was observed in the cochlea post-administration.
- BM-MSCs demonstrated good biocompatibility within the inner ear environment.
Conclusions:
- Non-surgical transtympanic delivery of BM-MSCs is safe in an immunocompetent cochlear model.
- This method holds potential for otoprotection against inner ear damage.
- Further exploration of BM-MSC therapy for cochlear insults is warranted.
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