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Illumina midi-barcodes: quality proof and applications
Julia Lanner1, Manuel Curto1, Bärbel Pachinger1
1a Institute for Integrative Nature Conservation Research , University of Natural Resources and Life Sciences , Vienna , Austria.
Mitochondrial DNA. Part A, DNA Mapping, Sequencing, and Analysis
|January 12, 2019
Summary
High-throughput sequencing (HTS) offers new DNA barcoding opportunities. Shorter DNA midi-barcodes from Illumina HTS effectively identified wild bees, overcoming some traditional DNA barcoding limitations.
Area of Science:
- Genetics
- Biodiversity Studies
- Bioinformatics
Background:
- DNA barcoding is a genetic tool for species identification.
- Traditional Sanger sequencing generates longer DNA barcodes (650 bp).
- High-throughput sequencing (HTS) presents new possibilities for genetic analysis.
Purpose of the Study:
- To evaluate the effectiveness of HTS for DNA barcoding.
- To compare DNA midi-barcodes generated by Illumina HTS and Sanger sequencing.
- To assess the utility of shorter DNA fragments for species identification.
Main Methods:
- Production of reference DNA midi-barcodes from wild bees.
- Utilizing both Illumina MiSeq and Sanger sequencing methods.
- Analysis of sequence data for species identification and comparison.
Main Results:
- Illumina HTS produced shorter DNA midi-barcodes (418 bp).
- Sequences from Illumina HTS were consistent with morphological species assignments.
- Illumina HTS barcodes were effective and addressed limitations of traditional DNA barcoding.
Conclusions:
- HTS is a viable method for DNA barcoding, particularly for shorter fragments.
- Illumina HTS provides an effective alternative for generating DNA barcodes for species identification.
- Shorter DNA midi-barcodes generated via HTS can overcome challenges in traditional DNA barcoding.
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