Toward an efficient antibacterial agent: Zn- and Mg-doped hydroxyapatite nanopowders

The use of synthetic hydroxyapatites (HAps) in biomedical and environmental applications is well warranted given that they have been shown to behave as an excellent bio-compatible material in human teeth and bones. In this paper, a series of HAps doped and co-doped with two metal cations (zinc and magnesium) has been successfully synthesized by means of the precipitation method using CaCl₂, Na₂HPO₄, ZnCl₂ and MgCl₂ aqueous solutions as reagents. The synthesized samples have been characterized using Fourier transform infrared (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and X-ray fluorescence spectroscopy (XRF). All samples prepared using over 10 mol% of Zn and Mg ions were identified as HAp. However, the presence of metal cations caused a significant increase in their crystallite sizes (30-50 nm) along with the appearance of a second phase (scholzite, whitlockite). The XRF spectra indicated the presence of Ca, P, Zn and Mg in the powders prepared with a high Metal/P ratio (1.7-2). The antimicrobial activity of these nanopowders has been tested in vitro against five bacteria (Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa as Gram-negative; Staphylococcus aureus and Bacillus subtilis as Gram-positive) and two fungal strains (Candida albicans and Aspergillus niger). The outcomes revealed that these nanopowders exhibited strong antimicrobial activity, starting at 15 mol% of Zn and/or Mg.