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Dissection of Saccharomyces Cerevisiae Asci
Published on: May 19, 2009
A physical method for separating Saccharomyces cerevisiae cells according to their ploidy
C Vágvölgyi1, J Kucsera, L Ferenczy
1Department of Microbiology, Attila József University, Szeged, Hungary.
Canadian Journal of Microbiology
|September 1, 1988
Summary
A new centrifugation method separates yeast cells by ploidy. This technique converts cells to protoplasts for gradient separation and regeneration, useful when markers are unavailable.
Area of Science:
- Microbiology
- Cell Biology
- Biotechnology
Background:
- Ploidy variation in Saccharomyces cerevisiae is common.
- Separating yeast cells by ploidy is crucial for genetic studies.
- Existing methods may require selectable markers.
Purpose of the Study:
- To develop a novel centrifugation technique for separating Saccharomyces cerevisiae cells based on ploidy.
- To provide a method for ploidy separation independent of selectable markers.
Main Methods:
- Utilized genetically marked Saccharomyces cerevisiae strains.
- Developed a centrifugation technique involving protoplast conversion.
- Employed Nycodenz density gradients for protoplast separation.
- Regenerated separated protoplasts to obtain distinct ploidy populations.
Main Results:
- Successfully separated Saccharomyces cerevisiae cells of different ploidy levels from exponential phase cultures.
- Demonstrated the efficacy of the protoplast conversion and gradient centrifugation method.
- Confirmed the viability and regeneration of separated yeast cells.
Conclusions:
- The developed centrifugation technique offers a robust method for ploidy-based cell separation in Saccharomyces cerevisiae.
- This method is particularly valuable in scenarios lacking suitable selectable markers.
- The technique has potential applications in yeast genetics and cell biology research.
