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Updated: Jan 30, 2026

Development of a Larval Zebrafish Infection Model for Clostridioides difficile
Published on: February 14, 2020
Genomic analysis of three Clostridioides difficile isolates from urban water sources
Daniela Numberger1, Thomas Riedel2, Gayle McEwen1
1Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315, Berlin, Germany.
Abstract:
We investigated inflow of a wastewater treatment plant and sediment of an urban lake for the presence of Clostridioides difficile by cultivation and PCR. Among seven colonies we sequenced the complete genomes of three: two non-toxigenic isolates from wastewater and one toxigenic isolate from the urban lake. For all obtained isolates, a close genomic relationship with human-derived isolates was observed.
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