Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Cell Specific Gene Expression

Cell Specific Gene Expression

The Equilibrium Binding Constant and Binding Strength

The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

Ligand Binding Sites

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...

Target Cell Response to Hormones

Target Cell Response to Hormones

Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Efficacy and safety of an intravenous amikacin-containing regimen as initial therapy in <i>Mycobacterium avium</i> complex pulmonary disease.

Journal of clinical tuberculosis and other mycobacterial diseases·2026

Same author

Anti-Ro-52 antibody is a risk factor for recurrence in interstitial lung disease with positive anti-aminoacyl tRNA synthetase antibody: a single-center, retrospective observational study.

BMC pulmonary medicine·2026

Same author

Clinical manifestations of adult patients requiring influenza-associated hospitalization in the pre-, during, and post-COVID-19 pandemic periods: An internet-based, prospective, multicenter, cohort study in Japan.

Respiratory investigation·2026

Same author

Carboxy-terminal 24-amino-acid peptide of integral membrane protein 2A is produced in the heart and stimulates atrial natriuretic peptide release.

Scientific reports·2026

Same author

Pulmonary Artery Sarcoma With Lung Metastasis.

Respirology case reports·2026

Same author

Enhancement of TIRF Imaging of 3D-Cultured Spheroids via Hydrostatic Compression Using a Balloon Actuator.

Micromachines·2026

Search research articles

Related Experiment Video

Updated: Jan 30, 2026

Cell Capture Using a Microfluidic Device

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

Microfluidic Device for Screening for Target Cell-Specific Binding Molecules by Using Adherent Cells.

Maho Kaminaga¹, Tadashi Ishida², Tetsuya Kadonosono³

¹Department of Mechanical Engineering, School of Engineering, Tokyo Institute of Technology, Kanagawa 226-8503, Japan. kaminaga.m.ab@m.titech.ac.jp.

|January 13, 2019

Summary

This study presents a novel microfluidic device for efficiently screening cancer cell-specific binding molecules. The device successfully isolates target molecules, minimizing non-specific binding for improved cancer diagnostics.

Keywords:

adherent cells cell homogenous dispersion structure microfluidic device pneumatic microvalve screening target cell-specific binding molecules

More Related Videos

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Published on: November 29, 2018

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

Published on: February 11, 2008

Related Experiment Videos

Last Updated: Jan 30, 2026

Cell Capture Using a Microfluidic Device

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Published on: November 29, 2018

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

Published on: February 11, 2008

Area of Science:

Biomedical Engineering
Microfluidics
Biotechnology

Background:

Developing methods for identifying target cell-specific binding molecules is crucial for disease diagnostics and therapeutics.
Existing screening methods often face challenges with specificity and efficiency.

Purpose of the Study:

To develop and evaluate a microfluidic device for high-throughput screening of molecules that bind specifically to target cancer cells.
To enable the isolation and collection of cancer cell-specific binding molecules.

Main Methods:

A microfluidic device utilizing micro pillar arrays for cell dispersion and pneumatic microvalves for channel control was designed.
Serially connected filter chambers with non-target cells were incorporated to minimize non-specific binding.
The device's performance was validated using N87 (target) and HeLa (non-target) cancer cell lines and fluorescently labeled antibodies (anti-HER2 and anti-integrin).

Main Results:

The microfluidic device effectively filtered non-target cell-binding molecules.
The device demonstrated high specificity, reducing non-target antibody binding to detection limits.
The system successfully trapped and collected target cell-specific antibodies (anti-HER2) from cancer cells.

Conclusions:

The developed microfluidic device offers an effective platform for screening and isolating target cell-specific binding molecules.
This technology has significant potential for advancing cancer diagnostics and drug discovery.
The integrated design enhances specificity and efficiency in molecular screening processes.