Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

5.7K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.7K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.0K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.0K
Genetics of Speciation02:16

Genetics of Speciation

21.1K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
21.1K
What is Population Genetics?01:25

What is Population Genetics?

64.7K
A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
64.7K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.1K
Overview
80.1K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.2K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel epitope-based diagnostic probes selected by phage display for the serological detection of HDV

Memorias do Instituto Oswaldo Cruz·2026
Same author

Association of sociodemographic factors, lifestyle behaviors, anthropometric measures, and cardiometabolic health markers with blood pressure in adolescents: a cross-sectional analysis.

PeerJ·2025
Same author

Identification of a rare copy number polymorphic gain at 3q12.2 with candidate genes for familial endometriosis.

Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia·2024
Same author

Preventive activity of <i>Copaifera langsdorffii</i> Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in <i>in vitro</i> and <i>in vivo</i> models.

Journal of toxicology and environmental health. Part A·2021
Same author

Focused screening reveals functional effects of microRNAs differentially expressed in colorectal cancer.

BMC cancer·2019
Same author

A High-Content Screening Approach to Identify MicroRNAs Against Head and Neck Cancer Cell Survival and EMT in an Inflammatory Microenvironment.

Frontiers in oncology·2019
Same journal

PBAE nanoparticle-mediated delivery of ASCL1 and NGN2 genes for astroglia-to-neuron reprogramming to remodel glial scar for spinal cord injury repair.

Stem cell research & therapy·2026
Same journal

Lipoaspirate-derived secretome restores redox homeostasis to attenuate pathological scar formation.

Stem cell research & therapy·2026
Same journal

Identification and enrichment of human retinal organoid-derived red/green cone-competent precursors with enhanced axon dynamics.

Stem cell research & therapy·2026
Same journal

PTGIS inhibited osteogenic and promoted angiogenic differentiation of bone marrow mesenchymal stem cells via endoplasmic reticulum stress mediated PI3K/AKT signaling.

Stem cell research & therapy·2026
Same journal

ITGA1 promotes osteogenic differentiation of human periodontal ligament stem cells via FAK-mediated PI3K-Akt activation.

Stem cell research & therapy·2026
Same journal

Therapeutic potential of mesenchymal stem cell-derived apoptotic vesicles in liver fibrosis: targeting the IGFBP3/PI3K-AKT axis via miR-409-3p.

Stem cell research & therapy·2026
See all related articles

Related Experiment Video

Updated: Jan 30, 2026

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.5K

Arrayed functional genetic screenings in pluripotency reprogramming and differentiation.

Rodrigo Alexandre Panepucci1,2, Ildercílio Mota de Souza Lima3,4

  • 1Laboratory of Functional Biology (LFBio), Center for Cell-Based Therapy (CTC), Regional Blood Center of Ribeirão Preto, Rua Tenente Catão Roxo, 2501, Ribeirão Preto, SP, CEP: 14051-140, Brazil. rapane@gmail.com.

Stem Cell Research & Therapy
|January 13, 2019
PubMed
Summary
This summary is machine-generated.

Understanding pluripotent stem cell (PSC) biology requires functional screens. This review covers arrayed screening approaches to systematically interrogate gene function in PSC reprogramming, differentiation, and state transitions.

Keywords:
Arrayed functional screeningsCell differentiationCellular reprogrammingFlow cytometryFluorescence microscopyHigh content screeningHuman embryonic stem cellsPluripotent stem cellsRNA interferencemicroRNA

More Related Videos

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
09:20

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Published on: January 8, 2017

27.9K
Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP
08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP

Published on: April 3, 2012

21.1K

Related Experiment Videos

Last Updated: Jan 30, 2026

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets
07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.5K
Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
09:20

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Published on: January 8, 2017

27.9K
Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP
08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP

Published on: April 3, 2012

21.1K

Area of Science:

  • Stem cell biology
  • Molecular genetics
  • Genomics

Background:

  • Pluripotent stem cells (PSCs) are crucial for research and therapy.
  • Understanding PSC states (Naïve, Primed) and transitions is key.
  • Transcriptome and miRNome data exist, but functional insights are limited.

Purpose of the Study:

  • To review arrayed screening studies on PSC function.
  • To discuss methods for interrogating gene function in PSCs.
  • To highlight future directions in stem cell research.

Main Methods:

  • Systematic review of arrayed screening studies.
  • Analysis of focused and genome-wide genetic libraries.
  • Discussion of population-level vs. cell-level readouts.
  • Evaluation of plate readers, flow cytometry, and high-content screening.

Main Results:

  • Arrayed screening approaches systematically interrogate gene function in PSCs.
  • Different screening methods offer distinct advantages and limitations.
  • High-content screening provides detailed cell-level insights.

Conclusions:

  • Functional screening is essential for advancing PSC research.
  • Integrating screening data will drive progress in stem cell therapy.
  • Future research should focus on refining screening techniques and data integration.