Related Experiment Video

Updated: Jan 30, 2026

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
10:06

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

Published on: August 3, 2017

10.4K

CDK6 coordinates JAK2 V617F mutant MPN via NF-κB and apoptotic networks

Iris Z Uras1, Barbara Maurer1, Harini Nivarthi2

  • 1Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Vienna, Austria.

Blood
|January 13, 2019
PubMed

Insights

Targeting CDK6 offers a novel therapeutic strategy for myeloproliferative neoplasms (MPNs). Its absence improves survival and reduces disease symptoms by impacting stem cell quiescence, NF-κB signaling, and apoptosis.

Area of Science:

  • Hematology
  • Oncology
  • Molecular Biology

Background:

  • Myeloproliferative neoplasms (MPNs) are often driven by the JAK2 V617F mutation.
  • Current JAK inhibitor therapies for MPNs lack curative potential and sustained response.
  • There is a critical need for novel therapeutic strategies targeting MPN pathogenesis.

Purpose of the Study:

  • To investigate the role of Cyclin-Dependent Kinase 6 (CDK6) in the evolution of MPNs.
  • To evaluate CDK6 as a potential therapeutic target for MPN treatment.

Main Methods:

  • Analysis of Cdk6 function in MPN models.
  • Assessment of CDK6 protein's impact on malignant stem cell quiescence, NF-κB signaling, and apoptosis.
  • Comparison of CDK6 functions with palbociclib, a CDK4/6 inhibitor.

Main Results:

  • Absence of Cdk6 significantly ameliorates clinical symptoms and prolongs survival in MPN models.
  • CDK6 regulates malignant stem cell quiescence, promotes NF-κB signaling, enhances cytokine production, and inhibits apoptosis.
  • CDK6's pathogenic functions in MPNs are largely independent of its kinase activity, as shown by palbociclib's ineffectiveness.

Conclusions:

  • CDK6 plays a critical role in MPN pathogenesis through kinase-independent mechanisms.
  • Targeting CDK6 presents a promising novel therapeutic approach for patients with MPNs.
  • These findings provide a strong rationale for developing CDK6-specific therapies for MPNs.

More Related Videos

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection11:57

Organotypic Cerebellar Cultures: Apoptotic Challenges and Detection

Published on: May 17, 2011

18.7K
Detection and Isolation of Apoptotic Bodies to High Purity12:17

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

11.4K

Related Concept Videos

Coordination Number and Geometry02:57

Coordination Number and Geometry

For transition metal complexes, the coordination number determines the geometry around the central metal ion. Table 1 compares coordination numbers to molecular geometry. The most common structures of the complexes in coordination compounds are octahedral, tetrahedral, and square planar.
19.0K
Coordination Compounds and Nomenclature02:54

Coordination Compounds and Nomenclature

In most main group element compounds, the valence electrons of the isolated atoms combine to form chemical bonds that satisfy the octet rule. For instance, the four valence electrons of carbon overlap with electrons from four hydrogen atoms to form CH4. The one valence electron leaves sodium and adds to the seven valence electrons of chlorine to form the ionic formula unit NaCl (Figure 1a). Transition metals do not normally bond in this fashion. They primarily form coordinate covalent bonds, a...
26.7K
Lattice Centering and Coordination Number02:33

Lattice Centering and Coordination Number

The structure of a crystalline solid, whether a metal or not, is best described by considering its simplest repeating unit, which is referred to as its unit cell. The unit cell consists of lattice points that represent the locations of atoms or ions. The entire structure then consists of this unit cell repeating in three dimensions. The three different types of unit cells present in the cubic lattice are illustrated in Figure 1.
Types of Unit Cells
Imagine taking a large number of identical...
11.5K
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.5K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.0K
Equations of Motion: Rectangular Coordinates and Cylindrical Coordinates01:21

Equations of Motion: Rectangular Coordinates and Cylindrical Coordinates

Understanding the motion of particles is a fundamental aspect of classical mechanics, and the choice of the coordinate system plays a pivotal role in unraveling the complexities of their dynamics.
When a particle moves relative to an inertial frame, the equations of motion can be expressed using rectangular components. If the motion is confined to the x-y plane, the equations having the x and y coordinates only can be used to simplify the mathematical representation.
However, when particles...
771