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CRISPR-Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics

Debarati Ghosh1, Prabhadevi Venkataramani1, Saikat Nandi1

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY USA.

Cancer Cell International
|January 15, 2019
PubMed

Insights

CRISPR-Cas9 gene editing precisely modifies DNA for biological research and disease modeling. This review covers its mechanism, applications in animal models, cancer immunotherapy, and future therapeutic delivery systems.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Genome editing enables precise DNA manipulation, crucial for understanding gene function.
  • CRISPR/Cas9, derived from bacterial immunity, is a versatile RNA-guided DNA endonuclease.
  • Its programmability makes it efficient, scalable, and indispensable for biological research.

Purpose of the Study:

  • To review the CRISPR-Cas9 gene-editing mechanism.
  • To explore CRISPR-screening methods and therapeutic applications.
  • To discuss clinical trials, limitations, and future directions for CRISPR-Cas9.

Main Methods:

  • Review of CRISPR-Cas9 gene-editing mechanism.
  • Analysis of CRISPR-screening methodologies.
  • Examination of therapeutic targeting in animal models and cancer immunotherapy.

Main Results:

  • CRISPR-Cas9 facilitates genetic engineering of animal models for disease mechanism studies.
  • It aids in elucidating molecular details for improved therapeutic outcomes.
  • The technology shows promise in cancer immunotherapy and ongoing clinical trials.

Conclusions:

  • CRISPR-Cas9 is a powerful tool for biological research and therapeutic development.
  • Ongoing research focuses on overcoming limitations for clinical applicability.
  • Developing effective delivery systems is key for advancing CRISPR-Cas9 in cancer therapeutics.
Keywords:
CAR-T therapyCRISPR–Cas9Clinical trialsDrug resistanceGene editingImmunotherapyPrecision medicineTargeted cancer therapyTumor heterogeneity

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