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Pre- and postcolumn derivatization chemistry in conjunction with HPLC for pharmaceutical analysis
N D Danielson1, M A Targove, B E Miller
1Department of Chemistry, Miami University, Oxford, Ohio 45056.
Journal of Chromatographic Science
|August 1, 1988
Summary
This study summarizes derivatization reactions for high-performance liquid chromatography (HPLC) analysis of diverse pharmaceutical compounds. It covers alkaloids, amines, antibiotics, and more, aiding in drug analysis.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- High-performance liquid chromatography (HPLC) is a key technique in pharmaceutical analysis.
- Derivatization reactions enhance the detectability and separation of various pharmaceutical compounds using HPLC.
Purpose of the Study:
- To provide a comprehensive summary of derivatization reactions for HPLC analysis.
- To cover a wide range of pharmaceutical compound classes.
Main Methods:
- Literature review of derivatization techniques.
- Classification of pharmaceuticals into categories: alkaloids, amines, antibiotics, barbiturates, carbonyl/carboxylic compounds, catecholamines, hydroxy compounds, steroids, sulfur compounds, and miscellaneous.
Main Results:
- Detailed descriptions of derivatization reactions applicable to each pharmaceutical class.
- Highlighting methods suitable for high-performance liquid chromatography.
Conclusions:
- Derivatization is crucial for effective HPLC analysis of diverse pharmaceuticals.
- This summary serves as a valuable resource for researchers and analysts in the field.