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The Genome Sequence of M228, a Chinese Isolate of Pseudomonas syringae pv. actinidiae, Illustrates Insertion Sequence

Joycelyn Ho1, George Taiaroa2, Margi I Butler1

  • 1Department of Biochemistry, University of Otago, Dunedin, New Zealand.

Microbiology Resource Announcements
|January 15, 2019
PubMed

Insights

The complete genome sequence of Pseudomonas syringae pv. actinidiae biovar 3 strain M228 from China was determined. Comparing its insertion sequence profile with a New Zealand isolate revealed insights into the evolution of these elements in biovar 3.

Area of Science:

  • Bacteriology
  • Genomics
  • Plant Pathology

Background:

  • Pseudomonas syringae pv. actinidiae causes kiwifruit bacterial canker.
  • Biovar 3 strains are a significant concern in kiwifruit production.
  • Understanding strain evolution is crucial for disease management.

Purpose of the Study:

  • To present the complete genome sequence of a Chinese biovar 3 strain (M228) of Pseudomonas syringae pv. actinidiae.
  • To compare the insertion sequence (IS) profile of M228 with a New Zealand isolate (ICMP18708).
  • To gain insights into the evolutionary history of IS elements within biovar 3.

Main Methods:

  • Whole-genome sequencing of the M228 strain.
  • Bioinformatic analysis of the M228 genome.
  • Comparative analysis of insertion sequence profiles between M228 and ICMP18708.

Main Results:

  • The complete genome sequence of the Chinese biovar 3 strain M228 was successfully obtained.
  • Distinct insertion sequence profiles were observed between the Chinese and New Zealand biovar 3 isolates.
  • The comparison provided valuable data on the distribution and potential mobility of IS elements.

Conclusions:

  • The genome sequence of M228 provides a valuable resource for studying Pseudomonas syringae pv. actinidiae biovar 3.
  • Insertion sequence element dynamics likely play a role in the evolution and diversification of biovar 3 strains.
  • Further research into IS element evolution can inform strategies for controlling kiwifruit bacterial canker.

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