Related Experiment Video
Updated: Jan 30, 2026
09:44
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
2.9K
Genomic Sequence of the Prophage-Free Lactococcus lactis Strain IL6288
Alexander Bolotin1, Anne Aucouturier1, Alexei Sorokin1
1MICALIS Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
Microbiology Resource Announcements
|January 15, 2019
Summary
Researchers sequenced the genome of Lactococcus lactis IL6288, a derivative lacking prophages. This study details the precise deletion of prophages and identifies minor genetic variations in the IL6288 strain.
Area of Science:
- Microbiology and Genomics
- Bacteriology
- Molecular Biology
Background:
- Lactococcus lactis is a key bacterium in dairy fermentation.
- Bacteriophages (prophages) can impact bacterial physiology and industrial applications.
- Strain IL1403 is a well-characterized Lactococcus lactis subsp. lactis strain.
Purpose of the Study:
- To report the complete genome sequence of IL6288, a prophage-free derivative of Lactococcus lactis IL1403.
- To confirm the precise deletion of all prophage sequences in the IL6288 genome.
- To identify any other genomic variations present in the IL6288 strain compared to its parent.
Main Methods:
- Whole-genome sequencing of the Lactococcus lactis IL6288 strain.
- Bioinformatic analysis to identify prophage sequences and other genetic variations.
- Comparative genomics to compare IL6288 with the parental strain IL1403.
Main Results:
- The genome sequence of IL6288 was successfully determined.
- Confirmation of the complete and precise deletion of all prophage elements.
- Identification of several single-nucleotide variations and an additional copy of the IS981 insertion sequence element.
Conclusions:
- The genome sequencing provides a valuable resource for understanding Lactococcus lactis subsp. lactis.
- The IL6288 strain is a confirmed prophage-free derivative with minimal other detected genomic alterations.
- The identified genetic variations appear to have a limited impact on the cell physiology of IL6288.
Related Concept Videos
02:02Genomics
40.6K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.6K
02:30Genomic Imprinting and Inheritance
37.2K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
03:21Genome Size and the Evolution of New Genes
9.1K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.1K
02:02Cis-regulatory Sequences
11.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
16.0K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.0K
01:19Thermal Strain
2.9K
Thermal strain is a concept that arises when we consider how temperature changes affect structures. Unlike the conventional assumption that structures remain constant under load, real-world scenarios often involve temperature fluctuations that can significantly impact these structures. Consider a homogeneous rod with a uniform cross-section resting freely on a flat horizontal surface. If the rod's temperature increases, the rod elongates. This elongation is proportional to the temperature...
2.9K