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Published on: July 5, 2017
Polyphenols as resistance modulators in Arcobacter butzleri
Vanessa Sousa1, Ângelo Luís1, Mónica Oleastro2
1CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Avenida Infante D. Henrique, 6200-506, Covilhã, Portugal.
Certain polyphenols, particularly stilbenes like resveratrol and pinosylvin, can inhibit efflux pumps in Arcobacter butzleri. This reduces antibiotic resistance and enhances antibiotic effectiveness against this emerging pathogen.
Area of Science:
- Microbiology
- Pharmacology
- Natural Products Chemistry
Background:
- Arcobacter butzleri is an emerging pathogen with increasing antibiotic resistance.
- Alternative strategies are needed to overcome resistance in A. butzleri infections.
Purpose of the Study:
- To investigate polyphenols as efflux pump inhibitors (EPIs).
- To evaluate polyphenol-antibiotic interactions for enhanced activity against A. butzleri.
Main Methods:
- Minimum Inhibitory Concentration (MIC) determination of polyphenols.
- Ethidium bromide accumulation assays to assess efflux pump inhibition.
- Checkerboard dilution tests for synergistic effects with antibiotics.
Main Results:
- Stilbenes (resveratrol, pterostilbene, pinosylvin) showed activity against A. butzleri.
- Polyphenols, especially stilbenes, inhibited efflux pumps and increased antibiotic efficacy.
- Polyphenol-antibiotic combinations demonstrated additive effects.
Conclusions:
- Some polyphenols, particularly stilbenes, act as efflux pump inhibitors.
- Resveratrol and pinosylvin show potential as resistance modulators against A. butzleri.
- Polyphenols offer a promising alternative to combat antibiotic resistance in A. butzleri.
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