Related Experiment Video
Updated: Jan 30, 2026

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators
Published on: April 4, 2016
Crowding and conformation interplay on human DNA G-quadruplex by ultraviolet resonant Raman scattering
Silvia Di Fonzo1, Cettina Bottari, John W Brady
1Elettra-Sincrotrone Trieste S. C. p. A., Science Park, Trieste, I 34149, Italy. silvia.difonzo@elettra.eu.
Abstract:
The G-quadruplex-forming telomeric sequence (TTAGGG)4TT was investigated by polarized Ultraviolet Resonance Raman Scattering (UVRR) at 266 nm. The presence of 40% poly(ethylene glycol) and the so-called "self-crowding" condition were used to induce the hybrid-to-parallel topology transition. Analysis of frequency shifts with temperature showed the role of several functional groups in the topological transitions and provides structural dynamical information. Circular dichroism under similar conditions was used as a reference. UVRR shed light on the effect of intramolecular interactions and of local and environmental dynamics in promoting different G-quadruplex topologies, induced by solution conditions or by temperature changes. Overall, these findings showed the enormous potential of this spectroscopy for G-quadruplex conformational studies.
More Related Videos
Related Concept Videos
Conformity
Resonance
Conformations of Butane
DNA Helicases
Conformations of Cycloalkanes
Conformations of Cyclohexane
The chair form is the most stable and derives its name from its resemblance to the “easy chair.” In the chair conformation, two carbon atoms are arranged out-of-plane — one above and one below, minimizing the torsional strain. In the chair form, the bond angle is very close to the ideal...

