CpGSTd3 is a lambda-Cyhalothrin Metabolizing Glutathione S-Transferase from Cydia pomonella (L.)

Area of Science:

• Entomology
• Molecular Biology
• Biochemistry

Background:

• The codling moth, Cydia pomonella, is a significant agricultural pest.
• Understanding insecticide resistance mechanisms is crucial for pest management.
• Glutathione S-transferases (GSTs) play a role in detoxification pathways.

Purpose of the Study:

• To investigate the role of the delta class GST gene, CpGSTd3, in lambda-cyhalothrin detoxification in C. pomonella.
• To characterize the expression patterns and enzymatic activity of CpGSTd3.

Main Methods:

• Real-time quantitative PCR (qPCR) was used to analyze CpGSTd3 gene expression.
• Expression levels were examined across different developmental stages and tissues.
• Lambda-cyhalothrin exposure was used to study gene induction.
• Recombinant CpGSTd3 was expressed in E. coli and tested for enzymatic activity against lambda-cyhalothrin and CDNB.

Main Results:

• CpGSTd3 exhibited ubiquitous expression in C. pomonella, with highest levels in larvae and lowest in eggs.
• The gene was most abundant in larval midgut and Malpighian tubules, and adult abdomens.
• Exposure to lambda-cyhalothrin induced CpGSTd3 transcript levels within 3 hours, followed by downregulation.
• Recombinant CpGSTd3 demonstrated catalytic activity against CDNB and metabolized lambda-cyhalothrin.

Conclusions:

• CpGSTd3 is likely involved in the metabolic detoxification of lambda-cyhalothrin in C. pomonella.
• The findings provide insights into insecticide resistance mechanisms in this pest.