Related Experiment Video

Updated: Jan 30, 2026

Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
10:32

Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination

Published on: August 24, 2015

14.1K

Irreversible oxidations of platelet proteins after riboflavin-UVB pathogen inactivation

G Sonego1, M Abonnenc2, D Crettaz2

  • 1Laboratoire de recherche sur les produits sanguins, recherche et développement produits, transfusion interrégionale CRS, Épalinges, Switzerland; Faculté de biologie et de médecine, université de Lausanne, Lausanne, Switzerland.

Transfusion Clinique Et Biologique : Journal De La Societe Francaise De Transfusion Sanguine
|January 15, 2019
PubMed

Insights

Pathogen inactivation treatment of platelets increases oxidative stress, impacting platelet function. Proteomics revealed specific protein oxidation changes affecting aggregation and shape post-treatment.

Area of Science:

  • Blood banking and transfusion medicine
  • Proteomics and mass spectrometry
  • Biochemistry and molecular biology

Background:

  • Pathogen inactivation technologies can alter platelet properties.
  • Reactive oxygen species generated during treatment may accelerate platelet storage lesion.
  • Proteomic studies are crucial for understanding treatment-induced protein alterations.

Purpose of the Study:

  • To investigate the impact of riboflavin/UVB pathogen inactivation on platelet proteome oxidation.
  • To identify specific oxidized proteins and their functional implications in treated platelets.

Main Methods:

  • Platelet concentrates were prepared and subjected to riboflavin/UVB pathogen inactivation.
  • Liquid chromatography-tandem mass spectrometry was used for shotgun proteomic analysis.
  • Site-specific oxidation of peptides was quantified at day 2 of storage.

Main Results:

  • Shotgun proteomics identified 2534 proteins and 9350 peptides, with 1714 oxidized.
  • Eighteen peptides were exclusively oxidized in treated platelets, while 3 were exclusively oxidized in controls.
  • Interference with proteins crucial for platelet aggregation and shape change (e.g., talin, vinculin) was observed.

Conclusions:

  • Riboflavin/UVB pathogen inactivation induces site-specific oxidation in platelet proteins.
  • Oxidative stress impacts key proteins involved in platelet aggregation and shape.
  • These findings provide insights into the mechanisms underlying altered platelet function after pathogen inactivation.
Keywords:
Concentré plaquettaireEspères réactives de l’oxygèneInactivation des pathogènesOxidationOxydationPathogen inactivationPlatelet concentrateProteomicsProtéomiqueROSRiboflavinRiboflavineShotgun

More Related Videos

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate08:25

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

Published on: April 6, 2022

2.3K
Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System12:40

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

29.5K

Related Concept Videos

X-Inactivation01:58

X-Inactivation

The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
41.7K
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
11.4K
Reversible and Irreversible Processes01:14

Reversible and Irreversible Processes

The thermodynamic processes can be classified into reversible and irreversible processes. The processes that can be restored to their initial state are called reversible processes. It is only possible if the process is in quasi-static equilibrium, i.e., it takes place in infinitesimally small steps, and the system remains at equilibrium However, these are ideal processes and do not occur naturally. An ideal system undergoing a reversible process is always in thermodynamic equilibrium within...
5.8K
Oxidation Numbers03:14

Oxidation Numbers

In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.6K
Pyruvate Oxidation01:15

Pyruvate Oxidation

After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
168.8K
Oxidation-Reduction Reactions03:11

Oxidation-Reduction Reactions

Oxidation–Reduction Reactions
75.6K