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Identification of potential target genes associated with the reversion of androgen-dependent skeletal muscle atrophy

Priscila de O Coelho1, Flavia A Guarnier2, Leonardo Bruno Figueiredo3

  • 1Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Brazil.

Archives of Biochemistry and Biophysics
|January 15, 2019
PubMed

Insights

Researchers identified novel anti-atrogene genes that reverse muscle wasting. These genes exclusively respond to testosterone replacement, offering new insights into muscle atrophy attenuation.

Area of Science:

  • Molecular Biology
  • Physiology
  • Genetics

Background:

  • Muscle wasting (atrophy) is linked to systemic diseases like diabetes, cancer, and kidney failure.
  • Atrogenes, a set of genes, are modulated during muscle atrophy.
  • Molecular mechanisms driving muscle atrophy reversion remain largely uncharacterized.

Purpose of the Study:

  • To investigate the transcriptional changes during muscle atrophy reversion.
  • To identify genes specifically involved in reversing muscle wasting.
  • To explore the role of testosterone in muscle regeneration at the molecular level.

Main Methods:

  • Utilized cDNA microarrays to analyze gene expression in androgen-sensitive Levator ani muscle (LA).
  • Compared gene expression profiles during castration-induced atrophy and testosterone replacement.
  • Identified differentially expressed genes and their response patterns.

Main Results:

  • Most differentially expressed genes acted as atrogenes, responding to castration.
  • Identified seven specific genes (APLN, DUSP5, IGF1, PIK3IP1, KLHL38, PI15, MKL1) that responded exclusively to testosterone replacement.
  • Proteins encoded by these seven genes are linked to atrophy reversion and cell growth regulation.

Conclusions:

  • Discovered a set of 'anti-atrogene' genes crucial for muscle atrophy reversal.
  • These anti-atrogenes are regulated by testosterone, highlighting its role in muscle regeneration.
  • Findings provide new molecular targets and perspectives for combating muscle wasting diseases.
Keywords:
And atrophy reversionAtrogenesFBXO32Muscle atrophy

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