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Genetic diversity of human parechoviruses in stool samples, Germany

Corinna Pietsch1, Uwe G Liebert1

  • 1Institute of Virology, Leipzig University, Leipzig, Germany.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
|January 15, 2019
PubMed

Insights

Human parechoviruses (HPeV) cause gastroenteritis, especially in infants. This study found diverse HPeV genotypes in stool samples, with HPeV-1B and HPeV-3 being most common and associated with severe illness.

Area of Science:

  • Virology
  • Infectious Diseases
  • Molecular Epidemiology

Background:

  • Human parechoviruses (HPeV) are common viral pathogens.
  • HPeV infections primarily affect infants and young children.
  • Gastroenteritis is a known clinical manifestation of HPeV.

Purpose of the Study:

  • To investigate the genetic diversity of circulating HPeV genotypes.
  • To identify HPeV strains in patients with infectious gastroenteritis.
  • To correlate HPeV genotypes with disease severity.

Main Methods:

  • Real-time RT-PCR for HPeV RNA detection in stool samples.
  • VP3/VP1 junction sequencing for HPeV genotyping.
  • Complete coding sequence analysis for uncommon strains.
  • Phylogenetic analysis of VP1 sequences.

Main Results:

  • HPeV RNA detected in 6.3% of samples, with a higher rate (8.8%) in children under two.
  • Predominant genotypes identified were HPeV-1B (47%) and HPeV-3 (35%).
  • Rare strains including HPeV-2, -4, -5, and -6 were also detected.
  • Severe disease was associated with HPeV-1B, -3, and -6 infections.

Conclusions:

  • This study highlights significant genetic diversity among HPeV strains.
  • HPeV-1B and HPeV-3 are key genotypes associated with severe gastroenteritis.
  • The findings contribute to understanding HPeV epidemiology and clinical impact.
Keywords:
DiarrhoeaGenetic diversityMolecular epidemiologyPhylogenetic analysisSepsis-like illnessViral gastroenteritis

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