Passive immune haemolysis -- a class specific antibody assay for use in the pig

An antibody assay is described based on the principle of complement mediated lysis of sheep red blood cells labelled with antigen. The technique provides a sensitive class specific assay enabling antibody in all three major immunoglobulin classes to be quantitated independently. The assay may be performed in tubes allowing precise measurement of antibody concentration, or in microtitre plates which provides a rapid estimation of antibody titre.