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Short-term exposure to carbamazepine causes oxidative stress on common carp (Cyprinus carpio)
Eloy Gasca-Pérez1, Marcela Galar-Martínez2, Sandra García-Medina2
1Cátedra CONACYT, Laboratory of Aquatic Toxicology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, AV. Wilfrido Massieu s/n y cerrada Manuel Stampa, Col. Industrial Vallejo., Ciudad de México, CP, 07700, Mexico; Laboratory of Aquatic Toxicology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, AV. Wilfrido Massieu s/n y cerrada Manuel Stampa, Col. Industrial Vallejo., Ciudad de México, CP, 07700, Mexico.
Abstract:
The aim of this research was to determine the bioconcentration factor and if subacute exposure to carbamazepine (2 mg L-1) modifies the oxidative state of liver, gills and brain of Cyprinus carpio. This was measured through the following biomarkers: hydroperoxide and protein carbonyl content, lipid peroxidation degree, as well as superoxide dismutase, catalase and glutathione peroxidase activity. Carbamazepine concentration in carp's tissue was also determined by liquid chromatography with a diode arrangement detector. An increase in lipid peroxidation degree, hydroperoxide and protein carbonyl content, and a decrease in the activity of the antioxidant enzymes (P < 0.05) with respect to control was observed. Also, there is an increase in the concentration of carbamazepina present in the organs with respect to the water in the system, which denotes bioconcentration of the drug. In conclusion, carbamazepine is bioconcentrated and produces oxidative stress on the common carp (C. carpio).
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