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Updated: Jan 30, 2026

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge
Published on: September 26, 2016
Rapid of cultivation dissimilatory perchlorate reducing granular sludge and characterization of the granulation
Pengna Yin1, Jianbo Guo1, Shumin Xiao1
1Tianjin Key Laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, Jinjing Road 26#, Tianjin 300384, PR China.
Abstract:
To remove high-strength perchlorate, dissimilatory perchlorate reducing granular sludge (DPR-GS) was first cultivated. Three identical UASB reactors were set up under different seed sludge and up-flow velocities (RAS: active sludge (AS) and constant up-flow velocities; RDGS: denitrifying granular sludge (DGS) and constant up-flow velocities; RDGS-f: DGS and fluctuating up-flow velocities). The AS in the RAS was completely granulated by day 117, while the DGS in the RDGS and RDGS-f were both shortened the granulation time to 99 days. In addition, the fluctuating up-flow velocity can better ensure rapid cultivation of DPR-GS. Removal of ClO4- loading rate with 7.20 kg/(m3·d) occurred in all three reactors. The results of extracellular polymeric substances (EPS) composition analysis indicated the polysaccharose (PS) promoted the formation of bio-aggregates, while the protein (PN) benefited the granulation of sludge. The analyses of the microbial communities indicated that Sulfurospirillum and Acinetobacter were the dominant dissimilatory perchlorate reducing bacteria.
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