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A smartphone-based system for fluorescence polarization assays.

Zijian Zhao1, Le Wei1, Mingfeng Cao2

  • 1Department of Electrical and Computer Engineering, Iowa State University, Ames, IA 50011, United States.

Biosensors & Bioelectronics
|January 15, 2019
PubMed
Summary

This study presents a smartphone-based sensor for fluorescence polarization (FP) analysis, enabling rapid detection of biomolecules. This portable platform offers accurate diagnostics for various applications, including in resource-limited settings.

Keywords:
Fluorescence polarization assayImmunoassayMobile sensorSmartphone

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Fluorescence polarization (FP) assays are crucial for rapid diagnostics due to their ability to detect ligand-analyte binding by measuring molecular mobility.
  • Existing FP detection methods often require specialized laboratory equipment, limiting their accessibility in resource-limited settings.
  • There is a need for portable, cost-effective, and accurate FP analysis platforms for widespread clinical use.

Purpose of the Study:

  • To develop and validate a smartphone-based sensor system for fluorescence polarization analysis of biomolecules.
  • To create a portable and accurate FP detection apparatus using readily available components like a smartphone and 3D-printed parts.
  • To demonstrate the system's capability for sensitive and specific biomolecular detection, including a competitive immunoassay.

Main Methods:

  • Implementation of a 3D-printed holder integrating a smartphone camera and polarization optics (polarization beam cube).
  • Simultaneous detection of parallel and perpendicular polarized fluorescence emissions using the smartphone's sensor chip.
  • Development of a custom software application for image analysis and computation of the degree of polarization.

Main Results:

  • The developed smartphone-based FP system accurately measured the degree of polarization, validated with dye molecules in solutions of varying viscosities.
  • A competitive FP immunoassay for Prostaglandin E2 successfully detected the analyte with a limit of detection of 1.57 ng/mL.
  • The system demonstrated high sensitivity and accuracy comparable to traditional FP methods.

Conclusions:

  • A novel, portable, and cost-effective smartphone-based fluorescence polarization assay platform has been successfully developed.
  • This technology offers accurate biomolecular analysis, suitable for rapid diagnostics in clinical settings and resource-limited environments.
  • The platform holds significant potential for detecting various biomarkers, toxins, and pathogens, enhancing global health accessibility.