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Updated: Jan 30, 2026

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
Published on: November 26, 2017
How microwaves can help to study membrane ageing
M Rabiller-Baudry1, L Paquin1, C Lepéroux1
1Univ Rennes, CNRS, ISCR (Institut des Sciences Chimiques de Rennes) - UMR 6226, Rennes, France.
Abstract:
This paper studies original protocols of rapid PES/PVP membrane NaOCl degradation allowing at reaching ageing states that are representative of industrial ageing. The long term objective is to propose basis for further fundamental studies aiming at the improvement of the impact of membrane ageing on behaviour in UF (fouling and cleaning mastering). The key of several protocols is the use of ageing acceleration thanks to microwave irradiation, either continuous or pulsed ones, that can be further associated (or not) with short ageing time in UF conditions. To evaluate the representativeness of obtained aged membranes, comparisons are achieved between pristine, voluntary laboratory aged membranes and an industrial membrane at the end of its service-life. Several physico-chemical analyses were used (ATR-FTIR, SEM-EDX, contact angle, SEC-HPLC). Evaluation of UF performances were made in UF of a model protein (Lysozyme, 14,300 g.mol-1). Finally, the proof of concept is done that conditions using MW exist to reach ageing state representative of industrial ageing.
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