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Diosgenin exerts its tumor suppressive function via inhibition of Cdc20 in osteosarcoma cells

Cheng Long1, Juan Chen2, Hua Zhou3

  • 1a Department of Orthopedics, West China Hospital, Sichuan University , Chengdu, Sichuan Province , China.

Cell Cycle (Georgetown, Tex.)
|January 15, 2019
PubMed

Insights

Diosgenin, a natural compound, effectively inhibits cancer cell growth and metastasis in osteosarcoma (OS) by targeting Cdc20 (cell division cycle 20 homologue). This suggests diosgenin as a potential non-toxic therapeutic agent for OS treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Osteosarcoma (OS) is an aggressive cancer common in young adults.
  • Cdc20 (cell division cycle 20 homologue) plays an oncogenic role in OS.
  • Existing Cdc20 inhibitors have side effects, necessitating the search for safer alternatives.

Purpose of the Study:

  • To investigate the potential of the natural compound diosgenin as a Cdc20 inhibitor in osteosarcoma cells.
  • To evaluate the therapeutic effects of diosgenin on OS cell behavior.

Main Methods:

  • Cell viability was assessed using MTT assays.
  • Apoptosis was analyzed via FACS.
  • Cell migration and invasion were evaluated using wound healing and Transwell assays.
  • Protein expression, including Cdc20, was determined by Western blotting.
  • Transfection assays were used to study the role of Cdc20.

Main Results:

  • Diosgenin significantly inhibited osteosarcoma cell growth and induced apoptosis.
  • Diosgenin exposure reduced cell migration and invasion.
  • Diosgenin was found to inhibit Cdc20 expression in OS cells.
  • Overexpression of Cdc20 counteracted the anti-proliferative and anti-invasive effects of diosgenin.

Conclusions:

  • Diosgenin demonstrates anti-cancer properties against osteosarcoma by inhibiting Cdc20.
  • Targeting Cdc20 with diosgenin presents a promising, potentially non-toxic therapeutic strategy for osteosarcoma treatment.
Keywords:
Cdc20Diosgeninapoptosiscell growthosteosarcoma

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