Fluorescein derived Schiff base as fluorimetric zinc (II) sensor via 'turn on' response and its application in live...

A novel Schiff base L composed of fluorescein hydrazine and a phenol functionalized moiety has been designed and prepared via cost-effective condensation reaction. The L is utilized for selective sensing of Zn²⁺ over other environmental and biological relevant metal ions in aqueous alcoholic solution under physiological pH range. The binding of Zn²⁺ to the receptor L is found to causes ~23 fold fluorescence enhancement of L. The 1:1 binding mode of the metal complex is established by combined UV-Vis, fluorescence, and HRMS (high-resolution mass spectroscopy) spectroscopic methods. The binding constant (K_a) for complexation and the limit of detection (LOD) of Zn²⁺ is calculated to be 2.86 × 10⁴ M^-1 and 1.59 μM, respectively. Further photophysical investigations including steady-state, time-resolved fluorescence analysis and spectral investigations including NMR (nuclear magnetic resonance), IR (infrared spectroscopy) suggest introduction of CHEF (chelation enhance fluorescence) with the suppression of CN isomerization and PET (photo-induced electron transfer) mechanism for the strong fluorescent response towards Zn²⁺. Finally, the sensor L is successfully employed to monitor a real-time detection of Zn²⁺ by means of TLC (thin layer chromatography) based paper strip. The L is used in the cell imaging study using African green monkey kidney cells (Vero cells) for the determination of exogenous Zn²⁺ by Immunofluorescence Assay (IFA) process.