Poly(azomethine-urethane) and zeolite-based composite: Fluorescent biosensor for DNA detection

Area of Science:

• Biotechnology
• Materials Science
• Analytical Chemistry

Background:

• Developing selective and sensitive biosensors is crucial for accurate DNA molecule determination.
• Polymer-based materials offer versatile platforms for biosensor development.
• Zeolites can be incorporated into polymer matrices to enhance molecular interactions.

Purpose of the Study:

• To develop a highly selective and sensitive fluorescent biosensor for DNA molecule determination.
• To investigate the role of zeolite in enhancing the polymer-DNA interaction.
• To optimize parameters affecting the biosensor's performance.

Main Methods:

• Fabrication of a fluorescent biosensor using poly(azomethine-urethane) and zeolite.
• Optimization of polymer concentration, pH, and incubation time.
• Determination of linear range and limit of detection (LOD) for DNA.
• Evaluation of selectivity through interference studies with amino acids, cations, and organic compounds.

Main Results:

• The developed biosensor exhibited high sensitivity and selectivity for DNA.
• Optimal conditions were established for polymer concentration, pH, and incubation time.
• A linear range of 2.50–25.00 nmol/L and an LOD of 0.095 nmol/L for DNA were achieved.
• The biosensor showed minimal fluorescence changes in the presence of interfering substances, confirming good selectivity.

Conclusions:

• A novel and effective fluorescent biosensor for DNA detection has been successfully developed.
• The incorporation of zeolite significantly enhances the performance of the poly(azomethine-urethane)-based biosensor.
• The biosensor demonstrates excellent selectivity and sensitivity, making it suitable for DNA determination.