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Updated: Jan 30, 2026

In vitro Investigation of the MexAB Efflux Pump From Pseudomonas aeruginosa
Published on: February 17, 2014
Clofazimine Exposure In Vitro Selects Efflux Pump Mutants and Bedaquiline Resistance
Nabila Ismail1, Remco P H Peters2,3, Nazir A Ismail2,4,5
1Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa nabilai@nicd.ac.za.
Abstract:
Six in vitro clofazimine-resistant spontaneous mutants obtained from a wild-type or pyrazinamide-resistant ATCC reference strain were selected to evaluate bedaquiline cross-resistance. The reverse was conducted for bedaquiline mutants. All clofazimine mutants harboring an rv0678 mutation displayed phenotypic cross-resistance. We observed the same for rv0678 bedaquiline mutants; however, atpE bedaquiline mutants showed no phenotypic cross-resistance. This confirms that upfront clofazimine usage may impact subsequent bedaquiline use due to a shared efflux resistance pathway.
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