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Updated: Jan 30, 2026

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
Published on: October 11, 2022
A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
Marco Castello1, Giorgio Tortarolo1,2, Mauro Buttafava3
1Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy.
Abstract:
Image scanning microscopy (ISM) can improve the effective spatial resolution of confocal microscopy to its theoretical limit. However, current implementations are not robust or versatile, and are incompatible with fluorescence lifetime imaging (FLIM). We describe an implementation of ISM based on a single-photon detector array that enables super-resolution FLIM and improves multicolor, live-cell and in-depth imaging, thereby paving the way for a massive transition from confocal microscopy to ISM.
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