Related Experiment Video
Updated: Jan 30, 2026
10:36
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Published on: August 3, 2018
12.6K
Author Correction: A pH-correctable, DNA-based fluorescent reporter for organellar calcium
Nagarjun Narayanaswamy1,2, Kasturi Chakraborty3,4, Anand Saminathan1,2
1Department of Chemistry, The University of Chicago, Chicago, IL, USA.
Nature Methods
|January 16, 2019
Summary
New fluorescent biosensors allow researchers to track calcium levels in specific endosome populations within beta cells. This advancement overcomes previous limitations in measuring cellular calcium dynamics.
Area of Science:
- Cell Biology
- Biochemistry
- Physiology
Context:
- Measuring intracellular calcium dynamics is crucial for understanding cell function.
- Previous methods using pH- or Ca2+-sensitive dyes or fluorescent-protein-based sensors had limitations in resolving calcium levels within specific endosome subpopulations.
- This study introduces an updated methodology to address these limitations.
Purpose:
- To update a published paper with a new reference detailing advanced fluorescent biosensors.
- To highlight the improved methodology for measuring calcium levels in beta-cell endosomes.
- To provide a more accurate understanding of calcium dynamics in cellular compartments.
Summary:
- The paper has been updated to include a new reference (ref. 18) on fluorescent biosensors for measuring calcium levels in beta-cell endosomes.
- The introduction was revised to incorporate these sensors, distinguishing them from earlier pH- or Ca2+-sensitive dyes.
- This update refines the description of methods used to study endosomal calcium, improving clarity and accuracy.
Impact:
- Enables more precise investigation of calcium signaling within distinct endosomal subpopulations.
- Facilitates a deeper understanding of beta-cell function and calcium-related diseases.
- Provides researchers with an improved tool for studying cellular calcium dynamics.
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