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Cytotoxicity of bracket identification dyes.

Fatih Celebi, Ahmet Altun, Ali Altug Bicakci

    The Angle Orthodontist
    |January 16, 2019
    PubMed
    Summary

    Bracket identification dyes can be cytotoxic. Some dyes showed cytotoxicity at higher concentrations (5 and 10 mg/mL), suggesting alternative methods for bracket identification may be needed in orthodontics.

    Area of Science:

    • Biomaterials Science
    • Dental Materials
    • Orthodontics

    Background:

    • Orthodontic fixed appliances utilize bracket identification dyes.
    • The potential cytotoxicity of these commonly used dyes is not fully understood.

    Purpose of the Study:

    • To investigate the cytotoxicity of bracket identification dyes used in orthodontic fixed appliances.
    • To evaluate dye cytotoxicity at varying concentrations.

    Main Methods:

    • Six bracket brands were selected, and their identification dyes were scraped.
    • Cytotoxicity testing was performed at 2.5 mg/mL, 5 mg/mL, and 10 mg/mL using a real-time cell analysis system.
    • Statistical analysis included one-way ANOVA and Tukey's post hoc test.

    Main Results:

    Keywords:
    Bracket identification dyeCytotoxicityReal-time cell analysis

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    • No cytotoxicity was observed for any of the six dyes at a 2.5 mg/mL concentration.
    • Cytotoxicity was detected in three brands at 5 mg/mL and in four brands at 10 mg/mL.
    • Significant differences in cytotoxicity were found within and between groups.

    Conclusions:

    • Certain bracket identification dyes exhibit cytotoxicity at elevated concentrations.
    • The findings suggest a need to explore alternative methods for bracket identification in orthodontic applications.
    • Further research into biocompatible identification methods is warranted.