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Spy vs. spy: selecting the best reporter for 19F NMR competition experiments
Guilherme Vieira de Castro1, Alessio Ciulli
1Division of Biological Chemistry and Drug Discovery, School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK. a.ciulli@dundee.ac.uk.
Abstract:
Systematic characterization of a series of fluorinated VHL ligands, varying binding affinity and position of the trifluoromethyl group, qualifies a spy molecule for competitive 19F NMR screening and reveals guiding principles to develop highly sensitive assays with low material consumption.
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