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Related Experiment Video

Updated: Jan 30, 2026

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Fast upconversion super-resolution microscopy with 10 μs per pixel dwell times.

Xingyun Peng1, Bingru Huang, Rui Pu

  • 1Centre for Optical and Electromagnetic Research, South China Academy of Advanced Optoelectronics, South China Normal University, 510006 Guangzhou, China. zhanqiuqiang@m.scnu.edu.cn.

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This study enhances multi-photon upconversion super-resolution microscopy by using a high Yb3+ doping strategy. This significantly boosts scanning speed for real-time biological imaging.

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Area of Science:

  • Biomedical imaging
  • Nanotechnology
  • Optical microscopy

Background:

  • Multi-photon upconversion super-resolution microscopy offers advantages like deep tissue imaging and low phototoxicity.
  • Current limitations include slow scanning speeds due to long lanthanide emission lifetimes and low brightness.

Purpose of the Study:

  • To overcome the speed limitations of upconversion super-resolution microscopy.
  • To enable faster imaging for capturing dynamic biological processes.

Main Methods:

  • Developed a facile strategy using a high ytterbium (Yb3+) sensitizer doping approach for upconversion nanocrystals.
  • Optimized nanocrystal properties to increase emission intensity and accelerate emission transients.

Main Results:

  • Achieved a significant increase in upconversion nanocrystal brightness and a substantial reduction in emission lifetime.
  • Enabled a 10 μs per pixel scanning speed for upconversion stimulated emission depletion super-resolution microscopy.
  • Maintained emission depletion efficiency.

Conclusions:

  • The high Yb3+ doping strategy effectively overcomes speed limitations in upconversion super-resolution microscopy.
  • This advancement paves the way for real-time observation of biological activities using this imaging technique.