[Characteristics of oxidative metabolism parameters at acute intoxication induced by surrogate alcohol]

The aim of this study was to estimate the oxidative metabolism parameters in blood of patients with acute intoxication of different severity induced by surrogate alcohol. The study involved 30 people (26-40 years old) with a diagnosis of acute intoxication induced by surrogate alcohol. Two groups of patients were formed. 1st group included 10 patients with moderate intoxication (the ethanol concentration in blood - 2.5-3.5 g/l); 2nd group included 20 patients with severe intoxication (blood ethanol concentration - 3.5-5.0 g/l). The control group included 15 persons without history of alcohol abuse. In blood plasma the levels of carbonyl protein products, dityrosine containing cross-linked products of oxidized proteins and malondialdehyde were detected. In erythrocytes the concentrations of reactive carbonyl protein products and membrane-binding hemoglobin were detected. Additionally the indicators of clinical chemistry (activity of transaminases, total bilirubin, creatinine, urea, total protein) in blood were detected. The data were processed using the EXCEL statistical software package (Mann-Whitney test). In blood plasma of 1st group patients malondialdehyde and oxidized proteins concentrations were higher in comparison with control ones by 40.4 (р<0.05) and 28.4%, respectively. In blood plasma of 2nd group patients those indicators were higher in comparison with control ones by 60.6 (р<0.05) and 95.2% (р<0.01). The significant decreasing of carbonyl protein products was fixed in blood plasma of both groups of patients compared with the control persons. In erythrocytes of patients the significant increasing of carbonyl protein products were fixed by 103.4 (р<0.05) and 95.8% (р<0.05) respectively compared with the control ones. Changing of oxidative stress indicators in blood took place earlier than the augmentation of clinical chemistry indicators. Our findings emphasized the involving of the different types of oxidized proteins in patterns of the alcohol surrogates implementing mechanisms.