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Related Experiment Video

Updated: Jan 30, 2026

Mating-based Overexpression Library Screening in Yeast
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Pathway Analysis of Fucoidan Activity Using a Yeast Gene Deletion Library Screen.

Monika Corban1, Mark Ambrose2, Joanne Pagnon3

  • 1School of Medicine, University of Tasmania; Hobart TAS 7001, Australia. Monika.Corban@utas.edu.au.

Marine Drugs
|January 17, 2019
PubMed
Summary
This summary is machine-generated.

Fucoidan from brown algae shows anti-cancer potential by affecting multiple cellular processes. This study used yeast gene deletion to reveal fucoidan

Keywords:
HCT-116Undaria pinnatifidafucoidanyeast gene deletion library

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Area of Science:

  • Marine Biotechnology
  • Molecular Biology
  • Cancer Research

Background:

  • Fucoidan, a sulfated polysaccharide from brown algae, exhibits anti-cancer properties.
  • Its precise mechanisms, particularly signaling pathways, are not fully understood.

Purpose of the Study:

  • To identify cellular pathways affected by fucoidan using a genetic screen.
  • To gain insights into the anti-cancer effects of *Undaria pinnatifida* fucoidan.

Main Methods:

  • Systematic screening of 4,733 *Saccharomyces cerevisiae* gene deletion strains for fucoidan-gene interactions.
  • Confirmation of findings using cell cycle analysis and DNA damage detection in human cells.

Main Results:

  • Fucoidan impacts diverse eukaryotic processes: RNA metabolism, protein synthesis, transport, carbohydrate metabolism, mitochondria, cell cycle, and DNA repair.
  • It reduced cancer cell survival, induced DNA damage, and caused G1-arrest in colon cancer cells, but not in fibroblasts.

Conclusions:

  • *Undaria pinnatifida* fucoidan has broad cellular effects in eukaryotes.
  • Fucoidan inhibits human cancer cell growth through multiple pathways, warranting further investigation.