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Updated: Jan 30, 2026

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
Published on: October 28, 2021
Identification and characterization of Fusarium sp. using ITS and RAPD causing fusarium wilt of tomato isolated from
Irom Manoj Singha1, Yelena Kakoty2, Bala Gopalan Unni3
1Department of Biotechnology, Asian Institute of Management and Technology, Guwahati 781 023, Assam, India.
Abstract:
Fusarium oxysporum which causes wilt is a serious pathogen. Fusarium isolates were isolated from Assam located in North East region of India. Morphological identification of Fusarium isolates was done using conidial and hyphal structures. Molecular identification of Fusarium isolates was done by amplifying the internal transcribed spacer (ITS) region of the conserved ribosomal DNA using primers ITS1 and ITS4. All the ITS sequences were compared for gaps and similarity. Further, characterization of random amplified polymorphic DNA (RAPD) was carried out using 40 primers. 15 primers that gave reproducible results were selected. RAPD was used to observe the relatedness among these isolates. Thus, it was concluded that molecular profiling using ITS is an indispensable method for identification studies.
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