Related Experiment Video
Updated: Jan 30, 2026

Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
Published on: July 10, 2015
Evaluation of the genetic basis of heavy metal resistance in an isolate from electronic industry effluent
Manasi1, N Rajesh2, Vidya Rajesh1
1Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, R.R. Dist, Telangana 500 078, India.
Abstract:
Halomonas BVR 1 isolated from an electronic industry effluent had high level of resistance to heavy metals like cadmium, lead, zinc and to various antibiotics. Minimum Inhibitory Concentration (MIC) of the strain toward cadmium and lead was found to be 200 mg L-1 and 400 mg L-1 respectively, while it could tolerate zinc up to 250 mg L-1 and chromium up to 150 mg L-1. The present study proved the genetic contribution of heavy metal resistance in this strain to be plasmid mediated. Isolation of the plasmid from Halomonas BVR 1 and its subsequent linearization with Bam H1 confirmed the presence of a plasmid of size >10 kb. Plasmid curing experiments affirmed plasmid mediated heavy metal resistance. Additionally, genetic transformation of a non metal resistant lab strain Escherichia coli and the cured strain of Halomonas BVR 1 with the isolated plasmid increased their metal tolerance level by 50% confirming the genetic determinant to be present in the plasmid.
More Related Videos
Related Concept Videos
Bonding in Metals
Metallic Solids
All metallic solids exhibit high thermal and electrical conductivity, metallic luster, and malleability....
Alkali Metals
Table 1: Properties of the alkali metals
Metal-Ligand Bonds
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
Properties of Transition Metals
Genetics of Speciation

