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Updated: Jan 30, 2026

Preparation of High-Quality Fermented Fish Product
Published on: August 23, 2019
Purification and characterization of lipase by Bacillus methylotrophicus PS3 under submerged fermentation and its
Pushpinder Sharma1, Nivedita Sharma1, Shruti Pathania1
1University of Horticulture and Forestry, Nauni, Solan, HP 173230, India.
Abstract:
Lipase production bacterial isolate was isolated from soil of service station and identified as Bacillus methylotrophicus PS3 by 16SrRNA with accession number |LN999829.1|. Lipase enzyme was purified by sequential methods of ammonium sulfate precipitation and Sephadex G-100 gel column chromatography. The molecular weight of purified enzyme was 31.40 kDa on SDS-PAGE. This purification procedure resulted in 2.90-fold purification of lipase with a 24.10% final yield. The purified lipase presented maximal hydrolytic activity at a temperature of 55 °C, and pH of 7.0. Lipase activity was stimulated by Triton X-100 and SDS with Mg2+ and Ca2+ metals employ a positive effect and outlast its stable in organic solvent i.e. methanol and ethanol.
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