Related Experiment Video
Updated: Jan 30, 2026

Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management
Published on: March 28, 2025
Application of response surface methodology to optimize the extracellular fungal mediated nanosilver green synthesis
Abdelmageed M Othman1, Maysa A Elsayed1, Ali M Elshafei1
1Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), 33 El Bohouth St., Dokki, 12622 Giza, Egypt.
Abstract:
This study aims to optimize the biosynthesis of nanosilver particles mediated by Trichoderma viride ATCC36838 using response surface methodology (RSM). Silver nanoparticles (AgNPs) were biosynthesized effectively in terms of the factors impacting silver ion (Ag+) reduction to metallic nanosilver (Ag0) using culture filtrate under shaking condition. The results of statistics calculations revealed that 2 mM silver nitrate and 28% (v/v) of culture filtrate at pH 7.0 for 34 h were the optimum values for AgNPs biosynthesis. The characterization of the produced AgNPs was conducted using electron microscopy, energy dispersive X-ray analysis, UV/visible spectrophotometry, and Fourier transform infrared spectroscopy. Round to oval AgNPs were detected with aspects of TEM within diameter range of 4-16 nm. The results of this study could help in developing a reliable ecofriendly, simple, and low cost process for microbial assisted AgNPs green synthesis especially with the continuous increase in its application fields.
Related Concept Videos
Response Surface Methodology
The process of RSM involves several key steps:
Cell-mediated Immune Responses
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Green Algae
Fungal Group Zygomycota
Applications of GIS: Disaster Management and Emergency Response

