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Related Concept Videos

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Continuing care describes the variety of health, personal, and social services provided over a prolonged period. The need for continuing care is increasing because people are living longer. Many people do not have families or others to care for them. Continuing care is mainly for patients who are disabled, functionally dependent, or suffering from a terminal disease. It is available within institutional settings or in homes. Examples include nursing centers or facilities, assisted living,...
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A function is continuous at a point a if three conditions are met: the function is defined at a, the limit of the function as x approaches a exists, and this limit equals the function’s value. Mathematically, this is written asThis definition ensures the graph of the function does not exhibit any breaks, holes, or jumps at that point. Discontinuities occur when any of these conditions fail. A removable discontinuity exists when the two-sided limit exists but the function is either...
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Related Experiment Video

Updated: Jan 30, 2026

High-throughput Flow-cytometry Measurement of Cellular Mechanotype Based on Rupture and Delivery of DNA Tension Probes into Cells
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High-throughput Flow-cytometry Measurement of Cellular Mechanotype Based on Rupture and Delivery of DNA Tension Probes into Cells

Published on: June 13, 2025

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A continuous-flow acoustofluidic cytometer for single-cell mechanotyping.

Han Wang1, Zhongzheng Liu, Dong M Shin

  • 1Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.

Lab on a Chip
|January 17, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a high-throughput acoustofluidic method for measuring cell biophysical properties. This technique enables rapid, single-cell analysis crucial for cancer biomarker discovery and prognosis.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Cancer Research

Background:

  • Cellular biophysical properties, like compressibility, correlate with disease progression, including cancer development and metastasis.
  • Cancer cell heterogeneity necessitates rapid, high-throughput single-cell analysis for biomarker assessment in cancer staging and prognosis.
  • Existing acoustofluidic methods for cell mechanotyping offer contactless measurement but suffer from low throughput due to no-flow requirements.

Purpose of the Study:

  • To develop a high-throughput, continuous flow-based acoustofluidic method for cell mechanotyping.
  • To enable rapid, single-cell resolution measurement of cell biophysical properties.
  • To provide a simple and low-cost approach for advanced cell analysis.

Main Methods:

  • Implementation of a continuous flow-based acoustofluidic system.
  • Utilizing acoustic waves to manipulate and measure cell properties in a flow environment.
  • Single-cell resolution analysis of cellular biophysical characteristics.

Main Results:

  • Demonstration of a high-throughput cell mechanotyping technique.
  • Successful measurement of cell biophysical properties at single-cell resolution under continuous flow conditions.
  • Preservation of simplicity and low-cost advantages compared to previous methods.

Conclusions:

  • The developed acoustofluidic method significantly enhances throughput for cell biophysical property analysis.
  • This technique is valuable for assessing cancer cell heterogeneity and potential as biomarkers.
  • The continuous flow approach overcomes limitations of previous low-throughput methods.