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Updated: Jan 30, 2026

High-throughput Flow-cytometry Measurement of Cellular Mechanotype Based on Rupture and Delivery of DNA Tension Probes into Cells
Published on: June 13, 2025
A continuous-flow acoustofluidic cytometer for single-cell mechanotyping
Han Wang1, Zhongzheng Liu, Dong M Shin
1Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
Abstract:
The biophysical properties of cells such as their compressibility have been found to be closely related to disease progression such as cancer development and metastasis. As cancer cells are heterogeneous, rapid and high-throughput evaluation of cell biophysical properties at single-cell resolution is needed to assess their potential as biomarkers for cancer staging and prognosis. Acoustofluidics has shown promise as a contactless method for accurately measuring cell biophysical properties; however, previously reported methods had relatively low throughput due to their requirement of no-flow conditions. This work presents a high-throughput continuous flow-based acoustofluidic cell mechanotyping method at single-cell resolution that retains the advantage of simplicity and low-cost.
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