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Related Experiment Videos

Smooth muscle cells transiently express NCAM.

R A Akeson1, J R Wujek, S Roe

  • 1Division of Basic Science Research, Children's Hospital Research Foundation, Cincinnati, OH 45229-2899.

Brain Research
|September 1, 1988
PubMed
Summary

Neural cell adhesion molecule (NCAM) is transiently expressed in rat smooth muscle during development, both in vitro and in vivo. This finding extends to all muscle types, revealing a broader role for NCAM in muscle development.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Neuroscience

Background:

  • Neural cell adhesion molecule (NCAM) is typically found on neurons.
  • NCAM expression is transient in skeletal and cardiac muscle during development.
  • Smooth muscle was previously thought to lack NCAM expression.

Purpose of the Study:

  • To investigate NCAM expression in rat smooth muscle cells in vitro and in vivo.
  • To determine the developmental expression pattern of NCAM in smooth muscle.
  • To compare NCAM expression levels in smooth muscle with other muscle and neuronal cell types.

Main Methods:

  • Immunofluorescence using monoclonal antibody 3F4.
  • Western blot analysis to detect NCAM polypeptides.
  • Northern blot analysis to detect NCAM mRNA.

Related Experiment Videos

  • Immunohistochemistry on cryostat sections of rat tissues.

    • Main Results:

    • NCAM is expressed on cultured rat aortic smooth muscle (A10, A7r5) and mouse BC3H1 cells.
    • Major NCAM polypeptide is 140 kDa, with a 120 kDa form present.
    • A 6.7 kb NCAM mRNA is detected, along with 4.3 and 2.9 kb forms.
    • NCAM is transiently expressed in vivo in smooth muscle of aorta, intestine, and bladder, peaking at embryonic day 17.

    Conclusions:

    • Rat smooth muscle expresses NCAM in vitro and transiently in vivo.
    • NCAM expression in smooth muscle is developmentally regulated.
    • All muscle types exhibit transient NCAM expression during development.