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A novel screening method for potential naringinase-producing microorganisms.

Satish V Patil1,2, Sunil H Koli1, Bhavana V Mohite1

  • 1School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, India.

Biotechnology and Applied Biochemistry
|January 17, 2019
PubMed
Summary

A new, simple plate assay using iodine vapors allows for effective screening of microorganisms for naringinase production. This method addresses a key research gap, enabling the discovery of more potent naringinase enzymes for industrial applications.

Keywords:
agar plate assayextracellulariodinenaringinase

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Area of Science:

  • Biotechnology
  • Enzyme Technology
  • Microbiology

Background:

  • Naringinase is crucial for various industrial applications, but research progress is hindered by a lack of efficient screening methods.
  • Existing screening methods are often complex or not universally applicable across different microbial types (bacteria, fungi, actinomycetes).

Purpose of the Study:

  • To develop a simple, effective, and broadly applicable plate assay for screening microorganisms that produce naringinase.
  • To overcome the limitations of current screening techniques and accelerate the discovery of novel naringinase enzymes.

Main Methods:

  • Development of a novel plate assay utilizing iodine vapor exposure.
  • Application of the assay for screening diverse microbial populations for naringinase activity.

Main Results:

  • The developed plate assay provides a simple and effective method for identifying naringinase-producing microorganisms.
  • This technique is applicable across different microbial genera, including bacteria, fungi, and actinomycetes.

Conclusions:

  • The new iodine-based plate assay effectively fills a technological gap in naringinase research.
  • This method facilitates the screening of a wider range of microorganisms, potentially leading to the identification of superior naringinase producers for industrial use.