Luteinizing hormone releasing hormone (LHRH) neuroterminals mapped using the push-pull perfusion method in the rhesus...

In vivo luteinizing hormone releasing hormone (LHRH) release was measured in conscious, ovariectomized rhesus monkeys using a push-pull cannula inserted into the stalk-median eminence, and the relationship between sampling location and LHRH release was examined. Within an individual animal in which multiple experiments were conducted with different cannula placements, LHRH pulse frequency was consistent. In contrast, LHRH pulse amplitude and mean LHRH release varied with cannula tip location in a pattern which reflected the anatomical distribution of LHRH-immunoreactive fibers described for the rhesus monkey. These results suggest that our push-pull perfusion method is reliable for the in vivo measurement of LHRH and perhaps other neuropeptides and/or neurotransmitters, as well.