Related Experiment Video

Updated: Jan 30, 2026

Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
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Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library

Published on: September 30, 2019

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Small Peptide Modulation of Fibroblast Growth Factor Receptor 3-Dependent Postnatal Lymphangiogenesis

David P Perrault1, Gene K Lee1, Sun Young Park1

  • 11 Division of Plastic and Reconstructive Surgery, Department of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, California.

Lymphatic Research and Biology
|January 17, 2019
PubMed
Abstract

Insights

Fibroblast growth factor receptor 3 (FGFR3) inhibition blocks 9-cis retinoic acid-induced lymphangiogenesis without affecting normal lymphatic endothelial cell function. This suggests FGFR3 plays a key role in promoting lymphatic vessel growth.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Developmental Biology

Background:

  • Fibroblast growth factor receptor (FGFR) family are transmembrane receptors crucial for development, cell function, and disease.
  • FGFR3 is implicated in 9-cis retinoic acid (9-cisRA)-induced lymphangiogenesis and lymphedema improvement.

Purpose of the Study:

  • To validate the efficacy of a novel small peptide FGFR3 inhibitor, peptide P3.
  • To elucidate FGFR3's role in 9-cisRA-induced lymphangiogenesis using peptide P3.

Main Methods:

  • In vitro studies assessed peptide P3's effect on lymphatic endothelial cell (LEC) proliferation, migration, and tubule formation.
  • In vivo studies evaluated peptide P3's impact on 9-cisRA-induced tracheal lymphangiogenesis.

Main Results:

  • Peptide P3 effectively inhibited FGFR3 phosphorylation.
  • FGFR3 inhibition by peptide P3 did not impede normal LEC proliferation, migration, or tubule formation.
  • Peptide P3-mediated FGFR3 inhibition blocked 9-cisRA-stimulated LEC proliferation, migration, tubule formation, and in vivo lymphangiogenesis.

Conclusions:

  • FGFR3 is not essential for basal LEC functions but plays a key role in 9-cisRA-induced lymphangiogenesis.
  • Peptide P3 demonstrates potential as a specific regulator of 9-cisRA-mediated lymphatic vessel growth.
Keywords:
9-cis retinoic acidFGFR3VSPPLTLGQLLSlymphangiogenesislymphatic endothelial celllymphedemapeptide inhibitor

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