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Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
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Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

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[Effect of Xinfeng Capsule on Beclin1/PI3K-AKT-mTOR of Adjuvant Arthritis Rats]

Ya-Li Wang, Jian Liu, Lei Wan

    Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi = Chinese Journal of Integrated Traditional and Western Medicine
    |January 17, 2019
    PubMed

    Insights

    Xinfeng Capsule (XFC) treatment in adjuvant arthritis (AA) rats improved joint inflammation by modulating autophagy-related genes and proteins. XFC normalized levels of pro-inflammatory cytokines and enhanced autophagy markers, reducing swelling and arthritis index.

    Area of Science:

    • Immunology and Molecular Biology
    • Pharmacology and Traditional Chinese Medicine

    Background:

    • Adjuvant arthritis (AA) is an inflammatory condition characterized by joint swelling and damage.
    • Autophagy dysfunction is implicated in the pathogenesis of inflammatory arthritis.
    • Understanding the molecular mechanisms of AA and potential therapeutic interventions is crucial.

    Purpose of the Study:

    • To investigate the expression levels of autophagy-related genes and proteins (Atg5, Atg7, Atg12, LC3-II, Beclin1) and inflammatory cytokines (IL-1β, TNF-α, IL-4, IL-10) in AA rats.
    • To evaluate the therapeutic effects of Xinfeng Capsule (XFC) on AA in rats.
    • To explore the impact of XFC on autophagy pathways and inflammatory responses in AA.

    Main Methods:

    • Forty-eight male SD rats were divided into control, AA model, Western medicine (leflunomide), and XFC treatment groups.
    • AA was induced in rats, followed by 30 days of treatment.
    • Body weight, toe swelling, arthritis index, and pathological changes were assessed.
    • mRNA and protein expression levels of autophagy markers and serum cytokine levels were measured.

    Main Results:

    • AA rats exhibited increased toe swelling, arthritis index, IL-1β, and TNF-α, with decreased IL-4, IL-10, and autophagy markers (Atg5, Atg12, LC3-II, Beclin1).
    • XFC treatment significantly reduced toe swelling, arthritis index, and pro-inflammatory cytokines (IL-1β, TNF-α).
    • XFC treatment increased autophagy markers (LC3-II, Beclin1) and anti-inflammatory cytokines (IL-4, IL-10), while decreasing PI3K, AKT, and mTOR expression.

    Conclusions:

    • The study suggests that decreased autophagy contributes to synovial cell proliferation and joint inflammation in AA rats.
    • Xinfeng Capsule demonstrates therapeutic potential in ameliorating AA by restoring autophagy and modulating inflammatory cytokine profiles.
    • XFC effectively improves arthritis index, reduces toe swelling, and modulates the expression of autophagy-related genes and proteins in AA rats.

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