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Growth Assays to Assess Polyglutamine Toxicity in Yeast
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Excised linear introns regulate growth in yeast.

Jeffrey T Morgan1,2,3, Gerald R Fink2,3, David P Bartel4,5,6

  • 1Howard Hughes Medical Institute, Cambridge, MA, USA.

Nature
|January 18, 2019
PubMed
Summary
This summary is machine-generated.

Excised introns in yeast stabilize under stress, functioning within the TOR growth-signalling network. These specific introns, when deleted, cause aberrant growth, highlighting their biological importance.

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Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • RNA Biology

Background:

  • Spliceosomal introns are non-coding RNAs usually degraded rapidly after excision.
  • The target of rapamycin complex 1 (TORC1) is a central regulator of cell growth and metabolism.

Purpose of the Study:

  • To investigate the fate and function of excised introns in Saccharomyces cerevisiae under stress conditions.
  • To identify the molecular mechanisms governing intron stabilization and their role in cellular signaling.

Main Methods:

  • Analysis of excised intron accumulation in yeast under saturated growth and TORC1 inhibition.
  • Characterization of stabilized introns' association with spliceosomal components.
  • Genetic manipulation (deletion and reintroduction) of specific introns to assess their impact on yeast growth and stress response.

Main Results:

  • A subset of 34 excised introns accumulates as linear RNAs under TORC1 inhibition or saturated growth.
  • Stabilized introns possess a short distance between the lariat branch point and 3' splice site, crucial for their stability.
  • Deletion of these introns impairs yeast fitness in saturated conditions and leads to aberrant growth under rapamycin treatment.

Conclusions:

  • Excised introns can be stabilized under specific stress conditions, revealing a previously unrecognized biological function.
  • These stabilized introns play a role in the TORC1 growth-signalling network in S. cerevisiae.
  • The findings suggest that excised spliceosomal introns have broader biological functions beyond their role in pre-mRNA splicing.